A high-throughput method for screening of rapamycin-producing strains of Streptomyces hygroscopicus by cultivation in 96-well microtiter plates.
Identifieur interne : 001856 ( Main/Exploration ); précédent : 001855; suivant : 001857A high-throughput method for screening of rapamycin-producing strains of Streptomyces hygroscopicus by cultivation in 96-well microtiter plates.
Auteurs : Zhi-Nan Xu [République populaire de Chine] ; Wen-He Shen ; Xi-Yang Chen ; Jian-Ping Lin ; Pei-Lin CenSource :
- Biotechnology letters [ 0141-5492 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Agar-agar.
- métabolisme : Candida albicans, Streptomyces.
- méthodes : Biotechnologie.
- pharmacologie : Antibactériens, Sirolimus.
- Dosage biologique, Facteurs temps, Fermentation, Mutation.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Agar.
- chemical , pharmacology : Anti-Bacterial Agents, Sirolimus.
- metabolism : Candida albicans, Streptomyces.
- methods : Biotechnology.
- Biological Assay, Fermentation, Mutation, Time Factors.
Abstract
A novel high-throughput cultivation method was developed to rapidly screen large numbers of rapamycin-producing mutants of Streptomyces hygroscopicus by duplicate culturing of isolates on the surfaces of agar-solidified 96 wells in microtiter plates. One copy of the cultures was used for the rapamycin bioassay and the other identical copy, representing potentially high yielding strains, was preserved for further study. By integrating 96-well solid cultivation and the bioassay, we screened more than 7000 isolates and found 10 high-yielding strains. From these, one mutant produced 420 mug rapamycin/ml, which was double the yield of parent strain used in the submerged fermentation process.
DOI: 10.1007/s10529-005-8463-y
PubMed: 16132865
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Wen He" uniqKey="Shen W" first="Wen-He" last="Shen">Wen-He Shen</name></author><author><name sortKey="Chen, Xi Yang" sort="Chen, Xi Yang" uniqKey="Chen X" first="Xi-Yang" last="Chen">Xi-Yang Chen</name></author><author><name sortKey="Lin, Jian Ping" sort="Lin, Jian Ping" uniqKey="Lin J" first="Jian-Ping" last="Lin">Jian-Ping Lin</name></author><author><name sortKey="Cen, Pei Lin" sort="Cen, Pei Lin" uniqKey="Cen P" first="Pei-Lin" last="Cen">Pei-Lin Cen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16132865</idno><idno type="pmid">16132865</idno><idno type="doi">10.1007/s10529-005-8463-y</idno><idno type="wicri:Area/Main/Corpus">001823</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001823</idno><idno type="wicri:Area/Main/Curation">001823</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001823</idno><idno 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Zhejiang</orgName></affiliation></author><author><name sortKey="Shen, Wen He" sort="Shen, Wen He" uniqKey="Shen W" first="Wen-He" last="Shen">Wen-He Shen</name></author><author><name sortKey="Chen, Xi Yang" sort="Chen, Xi Yang" uniqKey="Chen X" first="Xi-Yang" last="Chen">Xi-Yang Chen</name></author><author><name sortKey="Lin, Jian Ping" sort="Lin, Jian Ping" uniqKey="Lin J" first="Jian-Ping" last="Lin">Jian-Ping Lin</name></author><author><name sortKey="Cen, Pei Lin" sort="Cen, Pei Lin" uniqKey="Cen P" first="Pei-Lin" last="Cen">Pei-Lin Cen</name></author></analytic><series><title level="j">Biotechnology letters</title><idno type="ISSN">0141-5492</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agar (chemistry)</term><term>Anti-Bacterial Agents (pharmacology)</term><term>Biological Assay (MeSH)</term><term>Biotechnology (methods)</term><term>Candida albicans (metabolism)</term><term>Fermentation (MeSH)</term><term>Mutation (MeSH)</term><term>Sirolimus (pharmacology)</term><term>Streptomyces (metabolism)</term><term>Time Factors (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agar-agar (composition chimique)</term><term>Antibactériens (pharmacologie)</term><term>Biotechnologie (méthodes)</term><term>Candida albicans (métabolisme)</term><term>Dosage biologique (MeSH)</term><term>Facteurs temps (MeSH)</term><term>Fermentation (MeSH)</term><term>Mutation (MeSH)</term><term>Sirolimus (pharmacologie)</term><term>Streptomyces (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Agar</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Sirolimus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Agar-agar</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Candida albicans</term><term>Streptomyces</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biotechnology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Candida albicans</term><term>Streptomyces</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biotechnologie</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term><term>Sirolimus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Assay</term><term>Fermentation</term><term>Mutation</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dosage biologique</term><term>Facteurs temps</term><term>Fermentation</term><term>Mutation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel high-throughput cultivation method was developed to rapidly screen large numbers of rapamycin-producing mutants of Streptomyces hygroscopicus by duplicate culturing of isolates on the surfaces of agar-solidified 96 wells in microtiter plates. One copy of the cultures was used for the rapamycin bioassay and the other identical copy, representing potentially high yielding strains, was preserved for further study. By integrating 96-well solid cultivation and the bioassay, we screened more than 7000 isolates and found 10 high-yielding strains. From these, one mutant produced 420 mug rapamycin/ml, which was double the yield of parent strain used in the submerged fermentation process.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16132865</PMID><DateCompleted><Year>2005</Year><Month>12</Month><Day>30</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0141-5492</ISSN><JournalIssue CitedMedium="Print"><Volume>27</Volume><Issue>15</Issue><PubDate><Year>2005</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Biotechnology letters</Title><ISOAbbreviation>Biotechnol Lett</ISOAbbreviation></Journal><ArticleTitle>A high-throughput method for screening of rapamycin-producing strains of Streptomyces hygroscopicus by cultivation in 96-well microtiter plates.</ArticleTitle><Pagination><MedlinePgn>1135-40</MedlinePgn></Pagination><Abstract><AbstractText>A novel high-throughput cultivation method was developed to rapidly screen large numbers of rapamycin-producing mutants of Streptomyces hygroscopicus by duplicate culturing of isolates on the surfaces of agar-solidified 96 wells in microtiter plates. One copy of the cultures was used for the rapamycin bioassay and the other identical copy, representing potentially high yielding strains, was preserved for further study. By integrating 96-well solid cultivation and the bioassay, we screened more than 7000 isolates and found 10 high-yielding strains. 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MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year><Month>03</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2005</Year><Month>05</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2005</Year><Month>05</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>9</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>12</Month><Day>31</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>9</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16132865</ArticleId><ArticleId IdType="doi">10.1007/s10529-005-8463-y</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><noCountry><name sortKey="Cen, Pei Lin" sort="Cen, Pei Lin" uniqKey="Cen P" first="Pei-Lin" last="Cen">Pei-Lin Cen</name><name sortKey="Chen, Xi Yang" sort="Chen, Xi Yang" uniqKey="Chen X" first="Xi-Yang" last="Chen">Xi-Yang Chen</name><name sortKey="Lin, Jian Ping" sort="Lin, Jian Ping" uniqKey="Lin J" first="Jian-Ping" last="Lin">Jian-Ping Lin</name><name sortKey="Shen, Wen He" sort="Shen, Wen He" uniqKey="Shen W" first="Wen-He" last="Shen">Wen-He Shen</name></noCountry><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Xu, Zhi Nan" sort="Xu, Zhi Nan" uniqKey="Xu Z" first="Zhi-Nan" last="Xu">Zhi-Nan 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